the effect of different stabilizers on stability of horseradish peroxidase- bovine serum albumin-aflatoxin b1, a conjugated tracer for detection of aflatoxin b1 in immunoassay-based methods

aflatoxins are a group of fungal toxic metabolites, which are contaminated certain food commodities. elisa is one of the sensitive methods for detection of aflatoxins. preparation and stabilizing of a proper conjugated tracer for detection of aflatoxins is probably the main step for designing an elisa method. in current study, different stabilizers were applied to stabilize a newly prepared conjugated molecule, horseradish peroxidase-bovine serum albumin- aflatoxin b1 (hrp-bsa-afb1). stabilizing effects of six different stabilizers were compared during 10 months verification at room temperature and 6 weeks verification at 37°c. based on the results, it was concluded that trehalose-containing stabilizers especially those contain casein in their compositions show the best stabilizing effects on hrp-bsa-afb1 conjugated tracer.